Ion mobility spectrometry as a high-throughput technique for in vitro transdermal Franz diffusion cell experiments of
B Baert1, S Vansteelandt, B De Spiegeleer
1Drug Quality and Registration (DruQuaR) Group, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Ghent, Belgium.
Abstract:
Rapid, low-cost and sensitive analytical methods are needed to analyse the large number of samples that are generated when investigating the absorption profile of drugs through the skin using Franz diffusion cell experiments (FDC). The goal of this study was to evaluate the potential of ion mobility spectrometry (IMS) for the quantitative analysis of active pharmaceutical ingredients (API) in transdermal research. Ibuprofen was used as a model drug and the optimal IMS parameters were determined using a Doehlert experimental design. To assess the usefulness of the IMS method, FDC experiments using human skin were conducted, covering a concentration range of 0.32-69.57μg/ml. The resulting analytical samples were analysed using IMS and subsequently compared to HPLC as a reference method. No significant differences were found between the results obtained using both analytical methods, with a mean skin permeability coefficient (K(p)) value of 0.013cm/h. The combination of fast detection times, sensitivity, low costs and easy maintenance of IMS instruments makes this technique an attractive alternative for HPLC in this type of experiments.
More Related Videos
10:10Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study
Published on: August 15, 2016
07:05Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section
Published on: June 20, 2025
Related Concept Videos
In Vitro Drug Dissolution: Alternative Methods
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
In Vitro Drug Dissolution: Compendial Testing Models II
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
Passive Diffusion: Overview and Kinetics
When administered orally, drugs establish a substantial concentration gradient between the gastrointestinal (GI) lumen and the bloodstream, expediting their diffusion into...
