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Fading of auramine-stained mycobacterial smears and implications for external quality assurance
Jessica Minion1, Shubhada Shenai, Viral Vadwai
1Department of Epidemiology, McGill University, Montreal, Canada.
Abstract:
Light-emitting diode fluorescence microscopy is being scaled up for tuberculosis control, but fading of auramine-stained slides could compromise external quality assurance. We stored auramine-stained slides and reexamined them over time. Slides stored in all environments faded quickly, with significant changes in the proportion of positive slides in as little as 1 week.
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