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[Characteristics of Con A induced cells and their inhibitory activities in patients with lymphoma]
Y Wu1
1Institute of Oncology, Beijing.
Abstract:
Using five different cell lines as reactive cells, the proliferative inhibitory effect of Con A induced suppressive cells (CISC) was observed. The percentage of inhibition of MLA144 was 93.1%, K562 77.5%, Raji 62.5%, Molt-4 46.4%, and P388 25.9%. It is possible that K562 and MLA144 can be used as reactive cells instead of the peripheral blood mononuclear cells. Supernatants of CISC were also shown to be inhibitory, although less so than CISC. The inhibitory activity of CISC did not seem to be related to prostaglandin. However, its activity could be diminished by anti-T11 monoclonal antibody and complement. These results indicate that CISC is a T11-positive lymphocyte population. The inhibitory activity of CISC from 48 normal donors was determined by this modified method and compared with that of 22 patients with non-Hodgkin's lymphoma. The CISC activity of the patients was significantly lower than that of the normal subjects. Addition of exogeneous IL-2 to the culture enhanced the inhibitory activity in both groups.
Insights
Concanavalin A-induced suppressive cells (CISC) inhibit cell proliferation, with K562 and MLA144 cell lines showing high inhibition. CISC activity is lower in non-Hodgkin
Area of Science:
- Immunology
- Cell Biology
Context:
- Investigating the role of Con A-induced suppressive cells (CISC) in immune regulation.
- Evaluating the efficacy of various cell lines as reactive cells for assessing CISC function.
Purpose:
- To characterize the proliferative inhibitory effect of CISC on different cell lines.
- To determine the cell surface markers and factors involved in CISC-mediated inhibition.
- To compare CISC activity between healthy donors and patients with non-Hodgkin's lymphoma.
Summary:
- Concanavalin A-induced suppressive cells (CISC) demonstrated significant proliferative inhibition across five cell lines, notably MLA144 (93.1%) and K562 (77.5%), suggesting their potential as alternatives to peripheral blood mononuclear cells.
- CISC-derived supernatants retained inhibitory activity, though diminished. Inhibition was linked to T11-positive lymphocytes, independent of prostaglandin, and sensitive to anti-T11 monoclonal antibody and complement.
- Patients with non-Hodgkin's lymphoma exhibited significantly lower CISC activity compared to normal donors, with exogenous IL-2 enhancing inhibition in both groups.
Impact:
- Identifies T11-positive lymphocytes as key mediators of CISC-induced suppression.
- Highlights potential diagnostic or therapeutic implications for non-Hodgkin's lymphoma by revealing altered CISC function.
- Suggests K562 and MLA144 cell lines as viable alternatives for in vitro CISC activity assays.