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Chromosome Preparation From Cultured Cells
Published on: January 28, 2014
Cytogenetics of solid tumours
1Cytogenetics Laboratory, Department of Haematology, SydPath, St. Vincent's Hospital, Sydney, NSW, Australia. rlukeis@stvincents.com.au
Abstract:
The study of chromosome abnormalities in solid tumours provides valuable information for the diagnosis and prognostic stratification of a variety of tumour subtypes. Technical challenges are encountered in tissue culture, harvesting and finally the interpretation of complex chromosome abnormalities. Molecular cytogenetic studies complement karyotype analysis, but the ability to assess all chromosome abnormalities simultaneously underscores the value of conventional cytogenetic analysis. This chapter provides detailed methods for solid tumour cytogenetics from tissue culture to banding.
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