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Updated: Jun 3, 2026

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography
Published on: January 6, 2023
Size-exclusion high-performance liquid chromatography of proteins
1Department of Biochemistry, Oxford University, South Parks Road, Oxford, UK.
Abstract:
In chromatography, a solute partitions between a mobile and a stationary phase. Uniquely in size-exclusion chromatography, both phases have identical physicochemical properties; differential partition is effected by restricting the access of the solute to the stationary phase. At its simplest, the stationary phase is held inside pores with a distribution of sizes, and some pores are too small to permit the entry of larger molecules-hence size exclulsion (SE) (or the alternative gel permeation) chromatography.
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