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dsRNA uptake in adult Drosophila
Benjamin Obadia1, Maria-Carla Saleh
1CNRS URA 3015, Institut Pasteur, Viruses and RNAi group, Paris, France.
Methods in Molecular Biology (Clifton, N.J.)
|March 25, 2011
Abstract:
RNA interference (RNAi) is a conserved sequence-specific gene silencing mechanism that is induced by double-stranded RNA (dsRNA). The development of methods that allow internalization of dsRNA and concomitant silencing of the desired gene has not stopped since the first demonstration of RNAi in Caenorhabditis elegans. In this chapter, we describe how to introduce exogenous dsRNA into adult Drosophila in order to interfere with endogenous or viral gene expression.
