Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
Neurons: The Axon01:21

Neurons: The Axon

Axons are long, cytoplasmic processes of nerve cells capable of propagating electrical impulses known as action potentials. The cytoplasm or axoplasm of an axon contains neurofibrils, neurotubules, small vesicles, lysosomes, mitochondria, and various enzymes, all encased within the axolemma, the plasma membrane of the axon.
The axon attaches to the cell body at a cone-shaped elevation called the axon hillock. The initial part of the axon, closest to the hillock, is known as the initial segment.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiple mTOR RNA localization signals regulate subcellular protein synthesis and axonal growth.

bioRxiv : the preprint server for biology·2026
Same author

A mouse model of autosomal dominant spastic ataxia and myopathy caused by a mutation in <i>Tuba4a</i>.

bioRxiv : the preprint server for biology·2026
Same author

Evaluation of Nicotinamide Riboside in Prevention of Small Nerve Fiber Axon Degeneration and Promotion of Nerve Regeneration.

Journal of the peripheral nervous system : JPNS·2026
Same author

An axon-intrinsic loop restricts nerve regeneration through axonal protein synthesis.

bioRxiv : the preprint server for biology·2025
Same author

Acetylation of Axonal G3BP1 through ELP3 Accelerates Axon Regeneration.

bioRxiv : the preprint server for biology·2025
Same author

KHSRP-mediated decay of axonally localized prenyl-Cdc42 mRNA slows nerve regeneration.

PLoS genetics·2025

Related Experiment Video

Updated: Jun 3, 2026

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
07:06

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Published on: February 6, 2026

Profiling axonal mRNA transport.

Dianna E Willis1, Jeffery L Twiss

  • 1Department of Biology, Drexel University, Philadelphia, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 25, 2011
PubMed
Summary
This summary is machine-generated.

This study details a method for isolating neuronal axons to analyze messenger RNA (mRNA) content. This technique helps understand how localized protein synthesis in axons responds to external signals, crucial for neuronal function.

More Related Videos

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
11:24

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

Published on: June 17, 2015

Related Experiment Videos

Last Updated: Jun 3, 2026

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
07:06

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Published on: February 6, 2026

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
11:24

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

Published on: June 17, 2015

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • mRNA localization and localized protein synthesis are vital for spatially regulating protein levels within distinct subcellular domains.
  • Neuronal axons and dendrites function as independent domains capable of responding to extracellular stimuli via localized protein synthesis.

Purpose of the Study:

  • To discuss methodologies for isolating neuronal processes.
  • To provide a detailed method for isolating axons from dorsal root ganglion neurons using a modified Boyden chamber for mRNA content analysis.
  • To demonstrate how this method can identify mRNAs with altered axonal transport in response to extracellular stimuli.

Main Methods:

  • Isolation of neuronal processes.
  • Utilizing a modified Boyden chamber for axonal isolation from adult dorsal root ganglion neurons.
  • Analysis of axonal mRNA content.

Main Results:

  • A specific method for isolating pure neuronal axons is presented.
  • The method allows for the identification of specific mRNAs whose axonal transport is modulated by extracellular stimuli.
  • This approach facilitates the study of localized protein synthesis in axonal compartments.

Conclusions:

  • The described method enables the analysis of axonal mRNA content and transport.
  • Understanding axonal mRNA localization and translation is key to comprehending neuronal function and response to stimuli.
  • This technique provides a valuable tool for investigating the role of axonal protein synthesis in neuronal plasticity and health.