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Related Experiment Video

Updated: Jun 3, 2026

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

RNA-binding protein immunopurification-microarray (RIP-Chip) analysis to profile localized RNAs.

Alessia Galgano¹, André P Gerber

¹Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, ETH Zurich, Zurich, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)

|March 25, 2011

Summary

This summary is machine-generated.

RNA-binding proteins (RBPs) control gene expression post-transcriptionally. This protocol details RNA-binding protein immunopurification-microarray profiling (RIP-Chip) for identifying RBP-bound mRNAs genome-wide.

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Last Updated: Jun 3, 2026

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

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Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Published on: July 10, 2019

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Post-transcriptional gene regulation is essential for cellular function.
RNA-binding proteins (RBPs) play a critical role in modulating mRNA expression.
Understanding RBP-mRNA interactions is key to deciphering gene regulatory networks.

Purpose of the Study:

To provide a detailed protocol for genome-wide identification of mRNA targets of RBPs.
To introduce RNA-binding protein immunopurification-microarray profiling (RIP-Chip) as a systematic method.
To highlight the utility of RIP-Chip in studying RBP-RNA networks and their dynamics.

Main Methods:

Purification of endogenous RBP-mRNA complexes using specific antibodies.
Identification of associated RNAs via DNA microarrays (RIP-Chip).
Application of RIP-Chip to study mRNA transport and subcellular localization.

Main Results:

The RIP-Chip method enables systematic examination of RNA targets for RBPs.
This technique allows for the genome-wide identification of mRNAs regulated by specific RBPs.
RIP-Chip can identify mRNAs transported by RNP-motor complexes to specific cellular compartments.

Conclusions:

RIP-Chip is a powerful technique for dissecting post-transcriptional gene regulation.
Further development of this method can offer global insights into RBP-RNA networks.
Understanding these networks is crucial for comprehending cellular processes, development, and disease.