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Updated: Jun 3, 2026

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
Published on: July 23, 2017
Phenotype changes inherited by crossing pyrethroid susceptible and resistant genotypes from the cattle tick
G Aguilar-Tipacamú1, R Rosario-Cruz, Robert J Miller
1FMVZ-UNACH, Camino Ejido Emiliano Zapata Km. 8, Tuxtla Gutiérrez, Chiapas, Mexico.
A specific sodium channel gene mutation in Rhipicephalus (Boophilus) microplus ticks is strongly linked to pyrethroid resistance. This finding suggests a single genetic change can confer resistance to multiple pyrethroids, aiding in pest control strategies.
Area of Science:
- Veterinary Entomology
- Molecular Entomology
- Pest Genetics
Background:
- Pyrethroid resistance in Rhipicephalus (Boophilus) microplus poses a significant threat to cattle farming.
- Understanding the genetic basis of this resistance is crucial for developing effective control measures.
Purpose of the Study:
- To investigate the genetic basis of pyrethroid resistance in Rhipicephalus (Boophilus) microplus.
- To determine the correlation between a specific sodium channel gene mutation and pyrethroid resistance phenotype.
Main Methods:
- Dialelic crosses and backcrosses were performed between pyrethroid-resistant (RR) and susceptible (SS) tick strains.
- Larval Packet Test (LPT) was used to measure pyrethroid resistance.
- Allele-Specific PCR (AS-PCR) was employed for genotyping the sodium channel Phe-Ile substitution.
Main Results:
- A significant correlation was observed between the RR genotype and survival rates across all tested pyrethroids (cypermethrin, deltamethrin, flumethrin).
- Monogenic inheritance was supported for cypermethrin and flumethrin resistance.
- The Phe-Ile substitution in the sodium channel gene was identified as a key factor in pyrethroid resistance.
Conclusions:
- A single substitution in the sodium channel gene can confer resistance to pyrethroids as a class in Rhipicephalus (Boophilus) microplus.
- This genetic insight is vital for managing pyrethroid resistance in cattle ticks.
- Further research into combined resistance mechanisms is warranted.
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