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Related Concept Videos

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Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

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Related Experiment Video

Updated: Jun 3, 2026

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils
09:44

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

Published on: January 3, 2025

Diurnal variation in neutrophil function.

M Shiraishi1, K Suzuki, T Abe

  • 1Department of Hygiene, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan.

Environmental Health and Preventive Medicine
|March 25, 2011
PubMed
Summary
This summary is machine-generated.

Human neutrophil functions exhibit significant diurnal variations. Key immune responses like reactive oxygen species production and chemotaxis peak at different times of day, suggesting a circadian rhythm in immune cell activity.

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Area of Science:

  • Immunology
  • Chronobiology
  • Cellular Biology

Background:

  • Neutrophils are critical immune cells involved in host defense.
  • Understanding their functional patterns over a 24-hour period is essential for immunology.
  • Previous research has suggested potential daily fluctuations in immune responses.

Purpose of the Study:

  • To investigate the diurnal variations in specific neutrophil functions.
  • To assess the relationship between neutrophil activity, cell counts, and serum factors throughout a 24-hour cycle.

Main Methods:

  • Collected venous blood from 9 healthy males at 4-hour intervals over 24 hours.
  • Assessed neutrophil chemotaxis using a modified Boyden technique.
  • Measured reactive oxygen species (ROS) production and serum opsonic activity via luminol-dependent chemiluminescence.

Main Results:

  • Neutrophil ROS production and serum opsonic activity peaked during the day and decreased overnight.
  • Peak times for ROS production correlated negatively with neutrophil and segmented neutrophil counts.
  • Neutrophil chemotaxis showed distinct peaks around 6:00 AM and 6:00 PM.

Conclusions:

  • Significant diurnal variations exist in human neutrophil functions.
  • Circadian rhythms influence neutrophil's capacity for ROS production, chemotaxis, and serum opsonic activity.
  • These findings highlight the temporal dynamics of innate immune responses.