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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Glucose regulates RMI1 expression through the E2F pathways in adipose cells.

Akira Suwa1, Masayasu Yoshino, Takeshi Kurama

  • 1Pharmacology Research Labs, Drug Discovery Research, Astellas Pharma Inc., 21 Miyukigaoka, Tsukuba-shi, Ibaraki, 305-8585, Japan. akira.suwa@jp.astellas.com

Endocrine
|March 25, 2011
PubMed
Summary

RecQ-mediated genome instability 1 (RMI1) is linked to energy balance. Glucose stimulates RMI1 expression in adipocytes, potentially through E2F transcription factors, revealing a novel regulatory mechanism.

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Area of Science:

  • Molecular Biology
  • Metabolism
  • Cell Biology

Background:

  • RecQ-mediated genome instability 1 (RMI1) is a novel molecule implicated in energy homeostasis.
  • Previous studies suggest RMI1 expression in adipose tissue influences energy balance.
  • The precise mechanisms regulating RMI1 expression remain largely unknown.

Purpose of the Study:

  • To investigate the regulation of RMI1 expression during adipocyte differentiation.
  • To determine the effect of glucose stimulation on RMI1 expression.
  • To elucidate the role of transcription factors in glucose-induced RMI1 regulation.

Main Methods:

  • 3T3-L1 fibroblasts were differentiated into adipocytes.
  • RMI1 expression levels were measured under basal and glucose-stimulated conditions.
  • Small interfering RNA (siRNA) was used to knockdown E2F5 and E2F8 mRNA.

Main Results:

  • RMI1 expression significantly increased during 3T3-L1 adipocyte differentiation.
  • Glucose stimulation elevated RMI1 expression approximately eightfold.
  • Knockdown of E2F5 or E2F8 using siRNA attenuated the glucose-induced RMI1 up-regulation.

Conclusions:

  • RMI1 expression is modulated during adipogenesis.
  • Glucose significantly induces RMI1 expression in adipocytes.
  • E2F transcription factors (E2F5 and E2F8) play a role in the glucose-mediated regulation of RMI1.