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Updated: Jun 3, 2026
A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage
Published on: May 10, 2022
DNA damage in Mexican women with cervical dysplasia evaluated by comet assay
Elva I Cortés-Gutiérrez1, Martha I Dávila-Rodríguez, Estela A Zamudio-González
1Division of Genetics, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Universidad Autónoma de Nuevo León, Monterrey, Mexico. elvacortes@cibinmty.net
Objective:
To investigate the association between the progressive stage of cervical dysplasia and DNA damage by comet assay.
Study Design:
A hospital-based, unmatched, case-control study was performed. DNA damage levels (none, low, medium and high) in the cervical epithelial cells of 31 women (10 with low grade squamous intraepithelial lesions [LSIL], 10 with high grade [HSIL] and 11 with no cervical lesion) were evaluated using the comet assay.
Results:
A significant increase in medium DNA damage was observed in women with HSIL (17 +/- 8.9) relative to that in the control women (9 +/- 6.1). A significant increase in high DNA damage was also observed in women with LSIL (23 +/- 15.4) or HSIL (32 +/- 13.1) relative to that in the control women (12 +/- 7.9).
Conclusion:
These findings confirm that the grade of a cervical lesion correlates with the degree of genomic instability.
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