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Updated: Jun 3, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Synthesis of the 5'-Se-thymidine phosphoramidite and convenient labeling of DNA oligonucleotide
1Department of Chemistry, Georgia State University, Atlanta, Georgia 30303, USA.
Abstract:
The synthesis of the 5'-Se-derivatized thymidine and phosphoramidite is reported, and the Se-phosphoramidite was incorporated into DNA. Because of its high nucleophilicity, this 5'-Se-moiety permits convenient postsynthetic modification of oligonucleotides. The proof of principle was successfully demonstrated by incorporating fluorescein into DNA. It was also found that the 5'-dye-labeled Se-DNA can be recognized by DNA polymerase as an efficient primer. This novel strategy opens a new avenue for nucleic acid probe preparation and detection.
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