Portable device based on chemiluminescence lensless imaging for personalized diagnostics through multiplex...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Molecular Diagnostics

Background:

• Developing versatile analytical devices for simultaneous bioassays is crucial for personalized medicine.
• Existing methods often lack the sensitivity, speed, or portability required for comprehensive diagnostic panels.

Purpose of the Study:

• To develop and optimize a simple, versatile analytical device for performing multiple bioassays simultaneously.
• To demonstrate the device's capability for sensitive and rapid quantification of diverse analytes.

Main Methods:

• A microfluidics-based reaction chip with biospecific reactions and chemiluminescence detection was employed.
• A lensless, contact imaging configuration with a CCD sensor and fiber optic taper was utilized.
• Simultaneous quantification of alkaline phosphatase activity, Parvovirus B19 DNA, and horseradish peroxidase was performed.

Main Results:

• The device achieved high sensitivity, detecting proteins at attomole and nucleic acids at femtomole levels.
• Short analysis times (minutes) were realized due to miniaturized reaction chambers.
• Successful simultaneous quantification of three distinct targets demonstrated the system's multiplexing capability.

Conclusions:

• The developed device is a portable, sensitive, and versatile platform for multiplexed bioanalysis.
• It enables the creation of personalized diagnostic panel tests for early disease diagnosis and patient follow-up.
• This technology holds significant potential for advancing point-of-care diagnostics.