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Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
Published on: October 27, 2013
Development of a nested PCR for environmental detection of the pathogenic free-living amoeba Balamuthia mandrillaris
Arine F Ahmad1, Peter W Andrew, Simon Kilvington
1Department of Infection, Immunity & Inflammation, University of Leicester, Medical Sciences Building, PO Box 138, University Road, Leicester, LE1 9HN, United Kingdom.
The Journal of Eukaryotic Microbiology
|March 26, 2011
Abstract:
A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris ecology, geographic distribution, and public health risk.
