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Related Experiment Video

Updated: Jun 3, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

High throughput MLVA-16 typing for Brucella based on the microfluidics technology.

Riccardo De Santis¹, Andrea Ciammaruconi, Giovanni Faggioni

¹Histology and Molecular Biology Section, Army Medical and Veterinary Research Center, Rome, Italy. romano.lista@gmail.com

BMC Microbiology

|March 26, 2011

Summary

This study introduces a high-throughput microfluidics system for Brucella strain genotyping using MLVA-16 typing. The new method offers a faster and more efficient alternative to traditional techniques for accurate Brucella identification.

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Area of Science:

Microbiology
Genetics
Biotechnology

Background:

Brucellosis remains endemic globally, necessitating accurate strain typing for outbreak investigation and bioterrorism preparedness.
Multilocus Variable-number tandem repeat Analysis (MLVA-16) is a key genotyping technique for Brucella species.
Existing MLVA-16 methods include agarose gels, capillary electrophoresis, and microfluidic systems.

Purpose of the Study:

To describe and evaluate a high-throughput microfluidics system for MLVA-16 typing of Brucella.
To assess the accuracy and efficiency of the Caliper LabChip 90 system for Brucella genotyping.

Main Methods:

Evaluation of the Caliper LabChip 90 for MLVA-16 typing of 63 Brucella samples.
De novo genotyping of previously analyzed DNA samples to validate the system.
Comparison of MLVA typing data from Caliper system with sequencing and Agilent technologies.

Main Results:

The Caliper LabChip 90 showed good correlation with existing genotyping methods.
Discrepancies in DNA fragment sizes were observed, necessitating a conversion table.
The microfluidics system demonstrated high throughput and efficiency.

Conclusions:

The described MLVA-16 microfluidics system provides rapid and accurate Brucella strain genotyping.
The Caliper LabChip 90 offers significant improvements in handling, data acquisition, and speed compared to other microfluidic systems.
This platform is a valuable alternative for high-throughput Brucella genotyping, especially for large sample numbers.