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Published on: June 2, 2015
Inhibition of neuronal 5-HT uptake by ketamine, but not halothane, involves disruption of substrate recognition by
D C Martin1, R P Introna, R S Aronstam
1Department of Anesthesiology, Medical College of Georgia, Augusta 30912.
Abstract:
The effects of halothane and ketamine on (1) serotonin (5-hydroxytryptamine; 5-HT) uptake and (2) paroxetine binding to the 5-HT transporter in neuronal membranes were determined in rat brain. Both anesthetics inhibited the uptake of [3H]5-HT by synaptosomes, but only ketamine affected binding of [3H]paroxetine to the 5-HT transporter. Saturation analysis indicated that ketamine inhibition of [3H]paroxetine binding was competitive (Ki = 18.8 microM). These results indicate that halothane and ketamine inhibit 5-HT transport by different mechanisms.
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