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Updated: Jun 3, 2026

Dissection and Grading of Ovarian Development in Wild-Type Female Insects
Published on: July 14, 2023
Ovary development and maturation in Nezara viridula (L.) (Hemiptera: Pentatomidae)
P Fortes1, G Salvador, F L Cônsoli
1Depto de Entomologia e Acarologia, ESALQ, USP, Piracicaba, SP, Brasil.
Abstract:
Ovary development and maturation of Nezara viridula (L.) were evaluated by examining ovariole morphology and the alterations in the biochemical (protein synthesis related to reproduction) composition of the hemolymph. Quantitative and qualitative protein analyses were performed and ovary structural alterations for the pre-reproductive and reproductive stages were recorded. Total concentration of proteins in female hemolymph gradually increased until the end of the pre-mating stage, remaining unaltered thereafter. Proteins linked to reproduction (vitellogenins) appeared in the hemolymph 10 days after adult emergence and indicated the end of the pre-mating stage. After mating, total protein concentration in the hemolymph was lower compared to virgin females; vitellogenin levels were similar during most of the observation period. Oocyte development and maturation were gradual and age dependent. Ten-day-old females had chorionated oocytes ready for fertilization. Mating did not stimulate oocyte development in N. viridula, but the lack of mating activity appeared to have stimulated oocyte resorption in 17-day-old females.
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