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Related Experiment Video

Updated: Jun 3, 2026

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

Published on: August 26, 2014

Double-contrast micro-CT colonoscopy in live mice.

Hanne Boll¹, Simona Bag, Ingo S Nölte

¹Department of Neuroradiology Medical Faculty Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany.

International Journal of Colorectal Disease

|March 26, 2011

Summary

High-speed micro-computed tomography with double-contrast imaging enables effective colonoscopy in live mice. This technique successfully detects colonic polyps in murine models, aiding colon cancer research.

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Contrast Enhanced Vessel Imaging using MicroCT

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Last Updated: Jun 3, 2026

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

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Contrast Enhanced Vessel Imaging using MicroCT

Contrast Enhanced Vessel Imaging using MicroCT

Published on: January 27, 2011

Area of Science:

Biomedical Imaging
Gastroenterology
Oncology Research

Background:

Murine models are crucial for studying human colon cancer development.
Micro-computed tomography (micro-CT) is utilized for polyp detection in these models.
Faster scanning protocols are needed for enhanced colonoscopy in live subjects.

Purpose of the Study:

To evaluate a novel high-speed, continuous-rotation, single-breath-hold micro-CT protocol for colonoscopy in live mice.
To assess the feasibility of detecting colonic polyps using this rapid imaging technique.
To determine the effectiveness of double-contrasting the colon for improved visualization.

Main Methods:

C57BL/6JApcMin/+ mice underwent intubation and ventilation.
The colon was double-contrasted with barium and air.
Micro-CT scans were acquired during a 40-second single breath-hold using a continuous rotation protocol.
Polyp detection sensitivity was analyzed by blinded radiologists, and findings were verified post-excision.

Main Results:

A total of 12 polyps were detected in the explanted colons of six out of seven mice.
The sensitivity for detecting colonic polyps was 0.85 ± 0.1.
Tumors were located throughout the colon, with a mean volume of 6.5 ± 3.6 mm³.
The mean radiation dose was 0.241 ± 0.002 Gy.

Conclusions:

High-speed, continuous-rotation micro-CT with double-contrast is a feasible method for in vivo mouse colonoscopy.
The protocol provides sufficient contrast to visualize colonic folds and detect polyps.
This technique advances the study of colon cancer in murine models.