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Updated: Jun 3, 2026

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice
Published on: June 6, 2025
Adjuvant arthritis influences expression of housekeeping genes
Sherif Hanafy1, Fakhreddin Jamali
1Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2N8, Canada.
Objective And Method:
We determined whether adjuvant arthritis (AA) affects expression of three housekeeping (HK) genes, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), β-actin and 18s ribosomal RNA (18s rRNA) in organs of Sprague-Dawley rats using the real-time reverse-transcription polymerase chain reaction (RT-PCR).
Results:
Expression of 18s rRNA was unaffected by AA whereas that of β-actin was reduced by 52, 62 and 81% in the liver, kidney and intestine, respectively, but not in the heart. AA significantly reduced GAPDH expression in the intestine.
Conclusion:
For reliable results, the HK gene must be carefully selected.
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