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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
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Flow cytometry and immunoselection of human stem cells.

Terry E Thomas1, Sara J Abraham, Peter M Lansdorp

  • 1StemCell Technologies Inc., Vancouver, British Columbia, Canada.

Methods in Molecular Medicine
|March 26, 2011
PubMed
Summary

Isolating human hematopoietic stem cells (HSCs) from various tissues is challenging due to their low frequency. Effective purification requires multi-step cell separation techniques with high selectivity and capacity.

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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Cellular Immunology

Background:

  • Human hematopoietic stem cells (HSCs) are crucial for blood formation and can be sourced from bone marrow, peripheral blood, cord blood, and fetal liver.
  • Current isolation methods rely on cell properties like size, density, surface antigen expression, dye uptake, and drug sensitivity.

Purpose of the Study:

  • To address the challenge of isolating sufficient high-purity human hematopoietic stem cells (HSCs).
  • To review and highlight effective strategies for HSC purification.

Main Methods:

  • Fractionation of hematopoietic cell populations using techniques based on physical properties and cell-surface markers.
  • Multi-step cell-separation strategies employing varying capacities and selectivities.

Main Results:

  • The extremely low frequency of HSCs in source tissues poses a significant hurdle for purification.
  • Successful isolation of HSCs necessitates a combination of complementary cell-separation techniques.

Conclusions:

  • Effective purification of human hematopoietic stem cells (HSCs) demands sophisticated, multi-step approaches.
  • Optimizing cell-separation techniques is key to overcoming the challenge of low HSC frequency for research and clinical applications.