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Proteomic Profiling of Macrophages by 2D Electrophoresis
Published on: November 4, 2014
Proteomics in Ménière disease
Giuseppe Chiarella1, Milena Saccomanno, Domenica Scumaci
1Department of Experimental and Clinical Medicine, Audiology and Phoniatrics Unit, Magna Graecia University, Catanzaro, Italy. chiarella@unicz.it
Journal of Cellular Physiology
|March 26, 2011
Summary
This study identifies specific protein level changes in Ménière
Area of Science:
- Proteomics
- Biomarker Discovery
- Inner Ear Disorders
Background:
- Ménière's disease (MD) is an inner ear disorder with debilitating symptoms like vertigo and hearing loss.
- The exact pathophysiology of MD, including endolymphatic hydrops, remains largely unknown.
- Identifying reliable biomarkers for MD is crucial for early diagnosis and management.
Purpose of the Study:
- To employ a proteomics approach to identify potential plasma biomarkers for Ménière's disease.
- To compare plasma protein profiles of MD patients with those of healthy controls.
- To explore the potential of identified protein signatures for diagnostic and clinical applications.
Main Methods:
- Plasma samples were collected from 16 MD patients and healthy donors.
- High-abundance proteins were depleted to enhance detection of low-abundance proteins.
- Proteins were analyzed using two-dimensional gel electrophoresis and LC-MS/MS.
Main Results:
- Several proteins were found to be differentially expressed in MD patients.
- Overexpressed proteins include complement factors H and B, fibrinogen chains, beta-actin, and pigment epithelium-derived factor.
- Decreased levels were observed for beta-2 glycoprotein-1, vitamin D binding protein, and apolipoprotein-1.
Conclusions:
- The identified plasma protein profile may serve as a molecular signature for MD.
- This protein signature could offer a non-invasive tool for early diagnosis and clinical management of MD.
- Further research is needed to confirm the direct link to MD pathogenesis.
