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[Simultaneous determination of four alisols in Rhizoma Alismatis by RP-HPLC]
Zhangyan Luo1, Aicun Zhou, Chaofeng Zhang
1Research Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China.
Objective:
To develop an HPLC method for the determination of alisol A, alisol F, alisol A 24-acetate and alisol B 23-acetate in the rhizome of Alisma orientalis.
Method:
The separation was performed on a Shim-Pack CLC-ODS a C18 column (6 mm x 150 mm, 5 microm) eluted with the mobile phases of acetonitrile (A)-water (B) in gradient elution. The absorbance was monitored at 210 nm. Orthogonal test was adopted to optimize the extraction conditions of the four alisols.
Result:
The correlation coefficients of the four alisols were higher than 0.999 and the average recoveries were 99.23%, 96.67%, 97.30% and 99.61%, respectively. All the RSDs were less than 3%.
Conclusion:
The validation data demonstrated that the method was accurate and repeatable, and can be used to measure the four alisols in Rhizoma Alismatis.
