Related Experiment Video
Updated: Jun 3, 2026
A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
Published on: January 1, 2016
Preparation and characterization of an immunoaffinity column for the selective extraction of salbutamol from pork
Guomin Wang1, Yingguo Li, Xianliang Li
1Key Laboratory of Biorheological Science and Technology Under Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
Abstract:
A rapid, simple, and reliable determination method for salbutamol in pork was developed with immunoaffinity column (IAC) extraction followed by HPLC analysis. The salbutamol immunoaffinity column was prepared by coupling CNBr-activated Sepharose-4B with the anti-salbutamol polyclonal antibody which was purified by caprylic acid-ammonium sulfate. The coupling rate of the antibody and Sepharose-4B was 98.6%, and the dynamic column capacity of IAC was 400 ng/mL gel. The average recoveries of salbutamol from spiked pork samples at levels of 2, 10, 20, and 50 ng/g ranged from 83.3% to 92.2%, with the relative standard deviations of 2.8-7.0% (n=5), and the limits of detection and qualification were 0.25 ng/g and 0.5 ng/g, respectively.
