Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TLR7/8 agonist 3M-052 formulated in micelles induces local type I IFN response and antiviral immunity in zebrafish larvae.

Frontiers in immunology·2026
Same author

Emerging interest of anti-integrin αvβ6 antibodies for the diagnosis and prognosis of IBD and primary sclerosing cholangitis at the gut-liver interface.

Inflammatory bowel diseases·2026
Same author

Systems serology identifies FcR-related autoantibody signatures and functions for Sjögren's syndrome.

EMBO molecular medicine·2026
Same author

Current status of intranasal and inhaled COVID-19 vaccines.

NPJ vaccines·2026
Same author

Erratum: N1MΨU-modified mRNA vaccines break the mold in fish by enhancing innate immune activation.

Molecular therapy. Nucleic acids·2026
Same author

Postpandemic adjuvants to tailor vaccine-induced immunity.

Trends in immunology·2026

Related Experiment Video

Updated: Jun 3, 2026

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors
13:34

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

Published on: March 17, 2014

Phenotypic localization of distinct DC subsets in mouse Peyer Patch.

Nicolas Rochereau1, Bernard Verrier, Jean-Jacques Pin

  • 1GIMAP, EA3064, Faculté de médecine, Universités de Lyon, 42023 Saint-Etienne, France.

Vaccine
|March 29, 2011
PubMed
Summary

This study identifies novel mouse intestinal dendritic cell (DC) subsets in Peyer's patches. These findings enhance understanding of mucosal immunity and oral vaccine development.

More Related Videos

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches
09:49

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches

Published on: March 17, 2013

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
09:33

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

Published on: April 5, 2017

Related Experiment Videos

Last Updated: Jun 3, 2026

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors
13:34

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

Published on: March 17, 2014

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches
09:49

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches

Published on: March 17, 2013

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
09:33

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

Published on: April 5, 2017

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Peyer's patches are key inductive sites for intestinal mucosal immunity.
  • Dendritic cells (DCs) in the gut mucosa regulate immune responses, balancing protection and tolerance.
  • Existing research has described human mucosal DC subsets, but mouse mucosal DC subpopulations require further characterization.

Purpose of the Study:

  • To precisely characterize the phenotype and localization of dendritic cell subpopulations within mouse Peyer's patches.
  • To identify novel mouse intestinal DC subsets.
  • To provide insights into mucosal immune responses and oral vaccine evaluation.

Main Methods:

  • Immunofluorescence on cryosections and flow cytometry were employed.
  • Dendritic cells in C57Bl/6 mouse Peyer's patches were analyzed using specific DC markers.
  • Staining for CD11c, BDCA-2, pDC/IPC, DC-LAMP, DC-SIGN, TLR8, and Langerin was performed.

Main Results:

  • New mouse intestinal DC subsets were identified based on positive and double staining for various markers.
  • Specific phenotypes and localizations of these DC subsets within Peyer's patches were observed.
  • The study revealed distinct dendritic cell populations in the mouse gut.

Conclusions:

  • This research provides new insights into the heterogeneity of mouse mucosal dendritic cells.
  • Understanding these DC subsets is crucial for comprehending natural mucosal immune responses, such as to infections.
  • The findings offer new perspectives for the development and evaluation of oral vaccines.