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Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation
Published on: October 12, 2018
Polyanhydride microparticles enhance dendritic cell antigen presentation and activation
Maria P Torres1, Jennifer H Wilson-Welder, Senja K Lopac
1Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA.
Acta Biomaterialia
|March 29, 2011
Summary
Polyanhydride microparticles enhance immune responses by stimulating dendritic cells (DCs) and promoting antigen-specific T cell proliferation. This tailored approach offers potential for pathogen protection.
Area of Science:
- Biomaterials Science
- Immunology
- Nanotechnology
Background:
- Adjuvants are crucial for enhancing vaccine efficacy.
- Polyanhydride microparticles are biodegradable polymers with potential as vaccine delivery systems.
- Developing effective adjuvants without additional immune modulators is a key research area.
Purpose of the Study:
- To evaluate the adjuvant activity of polyanhydride microparticles without stabilizers, excipients, or immune modulators.
- To investigate the interaction of polyanhydride microparticles with dendritic cells (DCs).
- To assess the impact of polymer chemistry on immune cell activation and T cell responses.
Main Methods:
- In vitro culture of bone marrow-derived dendritic cells (DCs) with polyanhydride microparticles.
- Phagocytosis and intracellular localization studies of microparticles within DCs.
- Flow cytometry analysis of DC surface marker expression (MHC class II, CD86, CD40, CIRE).
- Cytokine secretion analysis (IL-12p40, IL-6).
- Antigen-specific T cell proliferation assays (CD4+ OT-II and CD8+ OT-I T cells) using ovalbumin-pulsed DCs.
Main Results:
- Polyanhydride microparticles were rapidly phagocytosed by DCs and localized to phagolysosomes with minimal erosion.
- Microparticle uptake increased DC surface expression of MHC class II, CD86, CD40, and CIRE.
- DC stimulation with microparticles enhanced secretion of IL-12p40 and IL-6 in a polymer chemistry-dependent manner.
- DCs treated with microparticles and ovalbumin induced antigen-specific proliferation of CD4+ and CD8+ T cells, dependent on polymer chemistry.
Conclusions:
- Polyanhydride microparticles demonstrate potent adjuvant activity by effectively stimulating dendritic cells.
- The immune response induced by these microparticles can be tailored by modifying polymer chemistry.
- These findings highlight the potential of polyanhydride microparticles as versatile platforms for inducing protective immunity against various pathogens.
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