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Receptor for Activated C Kinase-1 protein from Penaeus monodon (Pm-RACK1) participates in the shrimp antioxidant

Netnapa Saelee1, Moltira Tonganunt-Srithaworn, Warapond Wanna

  • 1Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand.

International Journal of Biological Macromolecules
|March 29, 2011
PubMed

Insights

Penaeus monodon Receptor for Activated C Kinase-1 (Pm-RACK1) demonstrates antioxidant functions, protecting cells and DNA from oxidative damage. This protein plays a role in shrimp antioxidant responses to reactive oxygen species (ROS).

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Marine Biology

Background:

  • Cellular oxidative stress, often caused by disease and environmental factors, necessitates effective antioxidant defense mechanisms for organism survival.
  • Reactive oxygen species (ROS) are key mediators of oxidative stress, requiring rapid elimination by cellular systems.
  • The role of Penaeus monodon Receptor for Activated C Kinase-1 (Pm-RACK1) in antioxidant pathways remains largely unexplored.

Purpose of the Study:

  • To investigate the potential antioxidant function of Penaeus monodon Receptor for Activated C Kinase-1 (Pm-RACK1).
  • To determine if Pm-RACK1 can protect cells and biomolecules from oxidative damage.
  • To explore the expression of Pm-RACK1 in shrimp under conditions known to induce oxidative stress.

Main Methods:

  • Overexpression of Pm-RACK1 in Escherichia coli and Spodoptera frugiperda (Sf9) insect cells followed by exposure to hydrogen peroxide (H2O2).
  • Purification of recombinant Pm-RACK1 protein expressed in E. coli.
  • Assay of the antioxidant activity of purified Pm-RACK1 against metal-catalyzed DNA oxidation.
  • Analysis of Pm-RACK1 gene expression in the hepatopancreas of Penaeus vannamei shrimp exposed to alkaline pH.

Main Results:

  • Overexpression of Pm-RACK1 conferred significant protection to E. coli and Sf9 cells against H2O2-induced oxidative damage.
  • Purified recombinant Pm-RACK1 exhibited antioxidant properties, protecting DNA from oxidation.
  • Shrimp exposed to alkaline pH, a condition known to increase ROS production, showed significantly higher Pm-RACK1 expression in their hepatopancreas compared to shrimp at pH 7.4.

Conclusions:

  • Pm-RACK1 possesses intrinsic antioxidant capabilities, protecting cellular components from oxidative stress.
  • The increased expression of Pm-RACK1 in shrimp under alkaline conditions suggests its involvement in the shrimp's antioxidant response to ROS.
  • Pm-RACK1 is a potential key player in mediating cellular defense against oxidative damage in crustaceans.

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