Related Experiment Video

Updated: Jun 3, 2026

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
11:28

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

Autographa californica multiple nucleopolyhedrovirus odv-e66 is an essential gene required for oral infectivity

Xingwei Xiang1, Lin Chen, Xiaolong Hu

  • 1College of Animal Sciences, Zhejiang University, Huajiachi Campus, Hangzhou 310029, China.

Virus Research
|March 29, 2011
PubMed

Insights

The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ODV-E66 protein is crucial for oral infection in P. xylostella larvae. However, this occlusion-derived virus (ODV)-specific envelope protein is not essential for viral replication or budded virus production.

Area of Science:

  • Virology
  • Molecular Biology
  • Insect Pathology

Background:

  • Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a baculovirus that infects insects.
  • The odv-e66 gene encodes ODV-E66, an occlusion-derived virus (ODV)-specific envelope protein.
  • The N-terminus of ODV-E66 targets proteins to viral envelopes during AcMNPV infection.

Purpose of the Study:

  • To investigate the role of the AcMNPV odv-e66 gene and its encoded ODV-E66 protein.
  • To determine the necessity of ODV-E66 for viral replication and infectivity.

Main Methods:

  • Construction of an odv-e66-knockout bacmid using homologous recombination in E. coli.
  • Assessing budded virus (BV) production and viral DNA replication in infected Sf9 cells.
  • Larval bioassays using P. xylostella, comparing injection and per os inoculation of wild-type, repaired, and odv-e66 deletion mutant viruses.

Main Results:

  • The odv-e66 deletion did not affect BV production or viral DNA replication in Sf9 cells.
  • Injection of odv-e66 deletion BV into P. xylostella hemocoel was as efficient as controls.
  • Per os inoculation of the odv-e66 deletion mutant showed a 10^3 higher 50% lethal dose compared to controls, indicating reduced oral infectivity.

Conclusions:

  • ODV-E66 is critical for the oral infectivity of AcMNPV in P. xylostella larvae.
  • ODV-E66 is not essential for AcMNPV replication or budded virus production.
  • The ODV-E66 envelope protein plays a significant role in AcMNPV pathogenesis via the oral route.

More Related Videos

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors06:02

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy09:13

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

Related Concept Videos

Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
Cytomegalovirus Disease01:27

Cytomegalovirus Disease

Cytomegalovirus (CMV) disease is caused by human cytomegalovirus, a double-stranded DNA virus of the Herpesviridae family. While primary CMV infection is often asymptomatic in immunocompetent individuals, the virus can cause severe disease in neonates and immunocompromised patients. CMV is the most common cause of congenital viral infection in the United States, and a major pathogen in solid organ and hematopoietic stem cell transplant recipients.CMV is transmitted via bodily fluids, sexual...
Inhibitors of Virion Maturation and Assembly01:19

Inhibitors of Virion Maturation and Assembly

As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the retrovirus to...