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Updated: Jun 3, 2026

Epithelial Cell Infection Analyses with Shigella
Published on: February 9, 2024
Multi-drug resistance and characteristic of integrons in Shigella spp. isolated from China
Jingyuan Zhu1, Guangcai Duan, Haiyan Yang
1Department of Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou 450001, Henan China.
Objective:
To investigate the characteristic of integrons and the relationship between integrons and antimicrobial resistance in Shigella spp.
Methods:
Ninety Shigella strains (83 S. flexneri and 7 S. sonnei) were isolated from the stools of patients in China. Susceptibility to 8 antimicrobials was tested for all isolated strains. PCR, RFLP and sequencing analysis of integrons were applied to all of them.
Results:
High prevalence of multi-drug resistance (95.6%) was identified. Of the isolates 79 (87.8%) carried integrase genes of class 1 integron (3.3%), class 2 integron (10.0%) or both (74.4%). No intI3 was detected in the tested isolates. The prevalence of intI2 was significantly higher in isolates with multi-drug resistance to at least 3 antibiotics than that in isolates with resistance to 2 and less antibiotics (P<0.05). Gene cassettes dfrA17-aadA5, dfrA12-orfF-aadA2 of class 1 integron and dfrA1-sat1-aadA1 of class 2 integron were identified.
Conclusion:
The class 2 integron may play a role in the emergence of multi-drug resistance in Shigella spp.
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