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Updated: Jun 3, 2026

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

Unique marker finder algorithm generates molecular diagnostic markers.

Sung-Kay Chiu¹, Ming-Hua Hsieh, Chi-Meng Tzeng

¹Department of Biology and Chemistry, City University of Hong Kong, Kowloon Tong, Hong Kong. kaychiu@cityu.edu.hk

International Journal of Bioinformatics Research and Applications

|March 29, 2011

Summary

This summary is machine-generated.

We developed Unique Marker Finder (U-MarFin) to identify unique DNA sequences for specific organisms. These sequences function as highly specific molecular biomarkers for diagnostic applications.

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Area of Science:

Genomics
Bioinformatics
Molecular Diagnostics

Background:

Comparative genomics offers powerful tools for identifying organism-specific genetic elements.
The need for precise molecular biomarkers in diagnostics is increasing.

Purpose of the Study:

To develop an algorithm for generating unique DNA sequences from a target organism.
To validate these sequences as specific molecular biomarkers for diagnostic purposes.

Main Methods:

Devised the Unique Marker Finder (U-MarFin) algorithm.
Partitioned genomes into fragments and eliminated homologous sequences using BLAST alignment.
Identified open reading frames as potential unique markers.
Validated sequence specificity using PCR and microarray technologies.

Main Results:

The U-MarFin algorithm successfully generated a collection of unique DNA sequences.
Empirical validation confirmed high species specificity of the discovered sequences.
The identified sequences demonstrated potential as molecular biomarkers.

Conclusions:

Unique Marker Finder (U-MarFin) is an effective tool for identifying unique genomic sequences.
The discovered sequences are highly specific and suitable for diagnostic applications.
This approach advances molecular diagnostics through comparative genomics.