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Quantification and evaluation of infectivity of shiga toxin-encoding bacteriophages in beef and salad
Lejla Imamovic1, Maite Muniesa
1Department of Microbiology, University of Barcelona, Diagonal 645, Annex, Floor 0, 08028 Barcelona, Spain.
Applied and Environmental Microbiology
|March 29, 2011
Abstract:
Stx bacteriophages in 68 samples of beef and salad were quantified by real-time quantitative PCR (qPCR). Stx phages from the samples were propagated in Escherichia coli C600, E. coli O157:H7, and Shigella strains and further quantified. Fifty percent of the samples carried infectious Stx phages that were isolated from plaques generated by lysis.

