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Related Experiment Video

Updated: Jun 3, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

Single molecule studies of DNA-protamine interactions.

Laura H Cree1, Rod Balhorn, Laurence R Brewer

  • 1The Voiland School of Chemical Engineering and Bioengineering, Center for Reproductive Biology, Washington State University, Pullman, WA 99164-2710, USA.

Protein and Peptide Letters
|March 30, 2011
PubMed
Summary
This summary is machine-generated.

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Correction to "High-Performance Concurrent Chemo-Immuno-Radiotherapy for the Treatment of Hematologic Cancer through Selective High-Affinity Ligand Antibody Mimic-Functionalized Doxorubicin-Encapsulated Nanoparticles".

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Erratum: Addition and Correction to "High-Performance Concurrent Chemo-Immuno-Radiotherapy for the Treatment of Hematologic Cancer through Selective High-Affinity Ligand Antibody Mimic-Functionalized Doxorubicin-Encapsulated Nanoparticles".

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Therapeutic applications of the selective high affinity ligand drug SH7139 extend beyond non-Hodgkin's lymphoma to many other types of solid cancers.

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New monoclonal antibodies specific for mammalian protamines P1 and P2.

Systems biology in reproductive medicine·2018

Single molecule studies reveal how protamine binds to DNA, forming sperm chromatin toroids. These interactions create DNA tension, influencing sperm nucleus shaping and volume reduction.

Area of Science:

  • Biochemistry and Molecular Biology
  • Cell Biology
  • Structural Biology

Background:

  • Sperm chromatin is highly condensed, utilizing protamine proteins instead of histones.
  • Understanding protamine-DNA interactions is crucial for male fertility and chromatin condensation.
  • Previous studies have offered insights into protamine-DNA complex morphology.

Purpose of the Study:

  • To review single molecule studies on protamine-DNA interactions.
  • To discuss the advantages of single molecule techniques in this field.
  • To present new measurements of DNA condensation forces induced by protamine.

Main Methods:

  • Single molecule force spectroscopy
  • Biophysical characterization of DNA-protamine complexes

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Last Updated: Jun 3, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
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Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

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Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

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  • Analysis of DNA condensation dynamics

    • Main Results:

    • Protamine binding kinetics and toroidal subunit morphology have been characterized.
    • Single molecule techniques offer advantages for studying dynamic interactions.
    • Protamine binding induces significant tension in DNA, impacting its structure.
    • New measurements quantify DNA condensation forces.

    Conclusions:

    • Protamine-DNA interactions are key to sperm chromatin condensation.
    • The induced DNA tension may play a role in spermatid nuclear shaping.
    • Single molecule studies provide critical insights into DNA-protamine complex stability and formation kinetics.