Related Experiment Video
Updated: Jun 3, 2026

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
Published on: January 10, 2025
ISsaga is an ensemble of web-based methods for high throughput identification and semi-automatic annotation of
Alessandro M Varani1, Patricia Siguier, Edith Gourbeyre
1Laboratoire de Microbiologie et Génétique Moléculaires, CNRS 118, Route de Narbonne, 31062 Toulouse Cedex, France. alessandro.varani@ibcg.biotoul.fr
Abstract:
Insertion sequences (ISs) play a key role in prokaryotic genome evolution but are seldom well annotated. We describe a web application pipeline, ISsaga (http://issaga.biotoul.fr/ISsaga/issaga_index.php), that provides computational tools and methods for high-quality IS annotation. It uses established ISfinder annotation standards and permits rapid processing of single or multiple prokaryote genomes. ISsaga provides general prediction and annotation tools, information on genome context of individual ISs and a graphical overview of IS distribution around the genome of interest.
Related Concept Videos
Genome Annotation and Assembly
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
In-situ Hybridization
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Tagging and Fusion Proteins

