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Updated: Jun 3, 2026
Quantification of Hypopigmentation Activity In Vitro
Published on: March 6, 2019
Inhibitory effect of a water extract from Pemphis acidula on melanogenesis in mouse B16 melanoma cells
Hsiou-Yu Ding1, Te-Sheng Chang, Chien-Min Chiang
1Institute of Cosmetics Science, Chia Nan University of Pharmacy and Science, National University of Tainan, Taiwan, ROC.
Abstract:
The inhibitory effect of a water extract from Pemphis acidula on melanogenesis in mouse B16 melanoma cells was investigated. The results showed that the P. acidula extract (PAE) inhibited melanogenesis in 3-isobutyl-1-methylxanthin (IBMX)-stimulated B16 cells in a dose-dependent manner, with an IC50 value of 33.5 μg/ml. In addition, PAE also inhibited cellular tyrosinase activity. Moreover, western blot and real-time reverse transcriptase polymerase chain reaction (qRT-PCR) analyses respectively confirmed that PAE down-regulated levels of tyrosinase protein and its mRNA in IBMX-stimulated B16 cells. These results demonstrated that PAE inhibits melanogenesis of B16 cells by reducing tyrosinase gene expression. From the present study, PAE is proven to be a good candidate as a skin-whitening agent for treatment of skin hyperpigmentation.
