Related Experiment Video
Updated: Jun 3, 2026
10:51
High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
Published on: December 5, 2016
Isolation and partial characterization of the Chlamydomonas reinhardtii γ-glutamyl kinase
Keita Miyoshi1, Hideyuki Matsuura, Takao Kumon
1Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Journal of Bioscience and Bioengineering
|March 30, 2011
Abstract:
We isolated a Chlamydomonas gene encoding putative γ-glutamyl kinase (GK), an enzyme that catalyzes the first step of proline biosynthesis. Using an Escherichia coli auxotroph and a purified recombinant protein, we show that Chlamydomonas GK is a functional GK. The sensitivity of this kinase to feedback inhibition by proline was lower than in that of microbial GKs previously reported.
