Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
Fermi Level Dynamics01:12

Fermi Level Dynamics

The vacuum level denotes the energy threshold required for an electron to escape from a material surface. It is usually positioned above the conduction band of a semiconductor and acts as a benchmark for comparing electron energies within various materials.
Electron affinity in semiconductors refers to the energy gap between the minimum of its conduction band and the vacuum level and it is a critical parameter in determining how easily a semiconductor can accept additional electrons.
The work...
Fast Reactions01:27

Fast Reactions

Fast reactions occurring in times shorter than the time needed to mix reactants pose a unique challenge for investigation. In a liquid-phase continuous-flow system, reactants A and B are swiftly pushed into the mixing chamber, where mixing occurs within 1 ms. The reaction mixture then flows through an observation tube, and one measures light absorption to determine species concentrations at various points of the tube. This method is most appropriate when relatively large volumes of reactants...
¹H NMR of Labile Protons: Temporal Resolution01:10

¹H NMR of Labile Protons: Temporal Resolution

Protons bonded to heteroatoms such as nitrogen and oxygen exhibit a range of chemical shift values. This is due to the varying degree of hydrogen bonding between the proton and the heteroatom in other molecules. The extent of hydrogen bonding affects the electron density around the proton, thereby giving different chemical shift values for the protons in the proton NMR spectrum.
The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the specific...
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How vibrational excitation shakes up the UV/VIS spectrum: A 2D-VE study.

The Journal of chemical physics·2026
Same author

Coherent two-dimensional electronic-x-ray spectroscopy.

The Journal of chemical physics·2026
Same author

Denoising and iterative phase recovery reveal low-occupancy populations in protein crystals.

Communications biology·2025
Same author

Optimization of vibrationally promoted electronic resonance (VIPER) excitation.

Physical chemistry chemical physics : PCCP·2025
Same author

Serial-femtosecond crystallography reveals how a phytochrome variant couples chromophore and protein structural changes.

Science advances·2025
Same author

XFEL Beamline Optical Instrumentation for Ultrafast Science.

The journal of physical chemistry. B·2024

Related Experiment Video

Updated: Jun 3, 2026

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data
08:12

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data

Published on: February 16, 2024

Modelling multi-pulse population dynamics from ultrafast spectroscopy.

Luuk J G W van Wilderen1, Craig N Lincoln, Jasper J van Thor

  • 1Division of Molecular Biosciences, Faculty of Natural Sciences, South Kensington Campus, Imperial College London, London, United Kingdom.

Plos One
|March 30, 2011
PubMed
Summary

This study introduces a MATLAB toolbox for analyzing molecular dynamics from ultrafast spectroscopy data. It enables systematic pathway analysis and resolves complex physical behaviors using advanced mathematical modeling.

More Related Videos

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers
09:49

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

Published on: October 23, 2018

Related Experiment Videos

Last Updated: Jun 3, 2026

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data
08:12

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data

Published on: February 16, 2024

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers
09:49

An Experimental Protocol for Femtosecond NIR/UV - XUV Pump-Probe Experiments with Free-Electron Lasers

Published on: October 23, 2018

Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Computational Chemistry

Background:

  • Advanced laser and electronic technologies enable sensitive recording of molecular dynamics.
  • Analyzing complex experimental data requires systematic investigation of connectivity schemes.

Purpose of the Study:

  • To present a MATLAB-based toolbox for analyzing molecular dynamics data.
  • To facilitate the extraction of physical pathways from experimental results.

Main Methods:

  • A toolbox with a graphical user interface for applying reaction models and generating differential equations.
  • Analysis of time-dependent datasets using gradient or direct search methods.
  • Inclusion of specific capabilities for ultrafast pump-probe spectroscopy, including chirp and instrument response function analysis.

Main Results:

  • The toolbox allows for the extraction of time-independent correlations from time-dependent datasets.
  • New theoretical models are incorporated to describe the orientation effects of intense polarized femtosecond pulses on particle ensembles.
  • The analysis accounts for realistic Gaussian beam profiles and includes corrections for anisotropy measurements.

Conclusions:

  • Mathematical modeling is essential for understanding and resolving the physical behavior of populations in ultrafast spectroscopy.
  • The developed toolbox provides a systematic approach to analyze complex molecular dynamics.
  • The study extends pathway modeling with new theory for polarized femtosecond pulse interactions.