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Mouse Adipose Tissue Collection and Processing for RNA Analysis
Published on: January 31, 2018
Molecular cloning, expression, and regulation of goose leptin receptor gene in adipocytes
Fang Wang1, Lizhi Lu, Hao Yuan
1College of Animal Sciences, Zhejiang University, 258 Kaixuan Road, Hangzhou, Zhejiang 310029, China.
Abstract:
A full-length cDNA encoding the goose (Anser anser) leptin receptor (LEPR) was cloned and sequenced. The goose LEPR gene encodes a 1,156-amino acid protein containing a signal peptide, a single transmembrane domain and specific motifs involving putative leptin-binding and signal transduction. The deduced goose LEPR protein shows more than 90% identity to duck and 75% identity to chicken and turkey. Quantitative real-time analysis reveals that the goose LEPR is predominantly expressed in brain. The expression of LEPR in goose adipocytes can be up-regulated by oleic acid in vitro. Moreover, the expression levels of genes, which have been demonstrated to be related to adipocyte differentiation, are down-regulated in LEPR-knockdown adipocytes, indicating LEPR's potential role in adipocyte differentiation in goose.
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