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Updated: Jun 3, 2026

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
Published on: April 19, 2013
[Q192R polymorphism of PON-1 gene in type 2 diabetic patients]
Abstract:
Polymorphism of PON-1 gene in 192 position of amino acid sequence of enzyme paraoxonase 1 was studied. For this research we have used the blood samples of 96 patients with T2DM and 123 healthy habitants of Kharkiv. Frequencies of alleles for the patients (pQ = 0,65, pR = 0,35) and the healthies (PQ = 0,70 and pR = 0,30) did not differentiate meaningfully. Distribution of the genotypes for healthy people does not correspond to Hardy-Weinberg equilibrium, the patients of T2DM have surplus of both homozygotes and shortage of heterozygotes. The risk of illness of T2DM for QQ homozygotes is 1,46-times higher and for QR heterozygotes is two times lower than on the average in the population (2%). The risk of illness of T2DM for RR homozygotes 1,86-times exceeds a middle population risk but is not significant.
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