Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nuclear Overhauser Enhancement (NOE)01:06

Nuclear Overhauser Enhancement (NOE)

Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...
Induced Electric Dipoles01:28

Induced Electric Dipoles

A permanent electric dipole orients itself along an external electric field. This rotation can be quantified by defining the potential energy because the external torque does work in rotating it. Then, the potential energy is minimum at the parallel configuration and maximum at the antiparallel configuration. While the former is a stable equilibrium, the latter is an unstable equilibrium.
Since the absolute value of potential energy holds no physical meaning, its zero value can be chosen as per...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Theory of Strong Electrolytes01:23

Theory of Strong Electrolytes

The interionic forces of the strong electrolytes depend on the solvent's dielectric constant, which is the ability of a solvent to store electrical energy, based on its polarizability. and the solution's concentration. In high-dielectric solvents and in dilute solutions, weak electrostatic forces keep ions apart. However, in low-dielectric solvents or concentrated solutions, stronger interionic forces may cause ions to pair up as ionic doublets despite being fully ionized. The theory of strong...
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-Brownian diffusion in lipid membranes: Experiments and simulations.

Biochimica et biophysica acta·2016
Same author

Nonergodicity, fluctuations, and criticality in heterogeneous diffusion processes.

Physical review. E, Statistical, nonlinear, and soft matter physics·2014
Same author

Structure-driven homology pairing of chromatin fibers: the role of electrostatics and protein-induced bridging.

Journal of biological physics·2013
Same author

Electrical monitoring of polyelectrolyte multilayer formation by means of capacitive field-effect devices.

Analytical and bioanalytical chemistry·2013
Same author

Detection of DNA hybridization by field-effect DNA-based biosensors: mechanisms of signal generation and open questions.

Biosensors & bioelectronics·2013
Same author

Polyelectrolyte adsorption onto oppositely charged interfaces: image-charge repulsion and surface curvature.

The journal of physical chemistry. B·2012

Related Experiment Video

Updated: Jun 3, 2026

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

DNA cyclization: suppression or enhancement by electrostatic repulsions?

A G Cherstvy1

  • 1Institute of Complex Systems, ICS-2, Theoretical Soft Matter and Biophysics, Forschungszentrum Jülich, Jülich, Germany.

The Journal of Physical Chemistry. B
|March 31, 2011
PubMed
Summary

Counterion condensation on polyelectrolyte rings enhances DNA cyclization. This study models ring formation, revealing conditions for electrostatically enhanced DNA ring formation, contrary to expectations.

More Related Videos

Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
10:35

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

Related Experiment Videos

Last Updated: Jun 3, 2026

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering
10:35

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

Area of Science:

  • Biophysics
  • Polymer Physics
  • Computational Chemistry

Background:

  • Polyelectrolytes are polymers with charged groups.
  • DNA cyclization is crucial for biological processes.
  • Counterion condensation influences polyelectrolyte behavior.

Purpose of the Study:

  • To model counterion condensation on polyelectrolyte rings.
  • To investigate the impact of electrostatic interactions on DNA cyclization.
  • To develop a theoretical framework for DNA ring formation probability.

Main Methods:

  • Analytical modeling of electrostatic energy for ring formation.
  • Computer simulations of short DNA fragments.
  • Incorporation of electrostatic energies for cyclization and dimerization.

Main Results:

  • Stronger counterion adsorption on rings than linear chains.
  • DNA cyclization probability can be electrostatically inhibited or enhanced.
  • Thermal fluctuations support electrostatically enhanced polyelectrolyte ring formation.

Conclusions:

  • Counterion condensation plays a key role in polyelectrolyte ring formation.
  • DNA cyclization can be enhanced by electrostatic effects under specific conditions.
  • Findings have implications for future DNA cyclization experiments and modeling.