Profiling of influenza viruses by high-throughput carbohydrate membrane array
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Updated: Jun 3, 2026
A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins
Published on: May 29, 2016
Weng-I Lao1, Ya-Fang Wang, Yu-Dai Kuo
1Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, No. 1, University Road, Tainan 70101, Taiwan.
Background:
Carbohydrate-protein interactions participate in many biological functions. To characterize the binding interactions represents a longstanding challenge.
Method:
We developed a glycan membrane array to study the interactions of carbohydrates with lectins, proteins and viruses, including 17 lectins, four antibodies (that are specific to different Lewis antigens), one recombinant H5N1 hemagglutinin, and five influenza B clinical isolates.
Results:
The results were found to be comparable to previous reports, indicating the efficacy and reliability of our developed method.
Conclusion:
This carbohydrate membrane array represents a convenient, reliable and low-cost method to examine the carbohydrate-binding features of various proteins, high-throughput drug screening and the glycan-binding surveillance of influenza viruses.
