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Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Transcription01:17

Transcription

Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription01:10

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

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Related Experiment Video

Updated: Jun 3, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

Developmental changes in transcriptional profiles.

William F Loomis1, Gad Shaulsky

  • 1Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA. wloomis@ucsd.edu

Development, Growth & Differentiation
|March 31, 2011
PubMed
Summary
This summary is machine-generated.

Global gene expression in Dictyostelium discoideum development was analyzed using RNA-sequencing. Researchers found that 65% of genes change significantly during development, with conserved patterns across species.

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Last Updated: Jun 3, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

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Published on: March 7, 2018

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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genomics

Background:

  • Global gene expression analysis provides insights into cellular processes.
  • Dictyostelium discoideum is a model organism for studying cellular development and differentiation.

Purpose of the Study:

  • To comprehensively map the developmental transcriptome of Dictyostelium discoideum.
  • To identify key genes and regulatory mechanisms governing Dictyostelium development.

Main Methods:

  • High-throughput sequencing of complementary DNA (RNA-seq) was employed to quantify mRNA abundance.
  • Transcriptional profiling of wild-type and mutant strains was performed at various developmental time points.
  • Comparative transcriptome analysis was conducted between Dictyostelium discoideum and Dictyostelium purpureum.

Main Results:

  • Approximately 65% of Dictyostelium genes exhibit twofold or greater changes in expression during development.
  • Gene expression patterns show dynamic changes, with many genes decreasing early and others increasing at specific stages.
  • Developmental gene expression patterns are conserved between Dictyostelium discoideum and Dictyostelium purpureum.
  • Key regulatory roles of DNA-binding proteins GBF and SrfA in developmental gene expression were identified.

Conclusions:

  • RNA-seq is a powerful tool for quantifying gene expression, even for low-abundance transcripts.
  • Dictyostelium development involves extensive and conserved transcriptional regulation.
  • Understanding these transcriptional dynamics is crucial for deciphering developmental pathways.