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Updated: Jun 3, 2026

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Published on: January 11, 2017
Expression profile and 5'-terminal structure of Arabidopsis antisense transcripts expressed in seeds
Masanori Okamoto1, Motoaki Seki
1RIKEN Plant Science Center, Yokohama, Kanagawa, Japan.
Abstract:
Advanced transcriptome analyses have revealed the existence of various RNA species. In our previous study, a large number of non-protein-coding RNAs including antisense transcripts were identified using an Arabidopsis tiling array. Most of the antisense transcripts exhibited co-expression with sense transcripts during stress treatments or seed imbibition. Here, we report that antisense transcripts exhibit differential expression patterns to sense transcripts in distinct developmental tissues. In addition, RNA ligase-mediated RACE analysis identified the existence of 5'-capped and -uncapped antisense transcripts. These observations provide additional insight into antisense transcripts.
