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Effect of phospholipids on UDP-glucose: ceramide glucosyltransferase from Golgi membranes
1Laboratoire de Biochimie des Membranes, Université Claude Bernard Lyon I, Villeurbanne, France.
The International Journal of Biochemistry
|January 1, 1990
Abstract:
1. The removal of phospholipids completely abolished the activity of the enzyme UDP-glucose:ceramide glucosyltransferase from Golgi membranes. 2. Modulation of enzyme activity by phospholipids was undertaken on the solubilized form of the enzyme. 3. Well-defined fatty acyl chains and polar head groups were necessary for maximal stimulation by phospholipids. 4. A specific requirement for phosphatidylcholine is suggested by preliminary experiments of reconstitution of enzyme activity with phosphatidylcholine vesicles.