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Updated: Jun 3, 2026
Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
Published on: August 22, 2017
Fluorimetric study on the interaction between Norfloxacin and Proflavine hemisulphate
Vishalkumar R More1, Prashant V Anbhule, Sang H Lee
1Department of Chemistry, Fluorescence Spectroscopy Research Laboratory, Shivaji University, Kolhapur 416 004 Maharashtra, India.
Abstract:
The interaction between Norfloxacin (NF) and Proflavine hemisulphate (PF) was investigated by spectroscopic tools like UV-VIS absorption and Fluorescence spectroscopy. It was proved that fluorescence quenching of NF by PF is due to the formation of NF-PF complex which was supported by UV-VIS absorption study. The study of thermodynamic parameters suggested that the key interacting forces are hydrogen bond and van der Waal's interactions and the binding interaction was spontaneous. The distance r between NF and PF was obtained according to the Förster's theory of non-radiative energy transfer. The fluorescence quenching mechanism was applied to estimate PF directly from pharmaceutical samples.
