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Updated: Jun 3, 2026
11:05
Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
Published on: February 21, 2019
Endocytic sorting of transmembrane protein cargo
Bernard T Kelly1, David J Owen
1Cambridge Institute for Medical Research, Dept of Clinical Biochemistry, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK. btk1000@cam.ac.uk
Current Opinion in Cell Biology
|April 1, 2011
Summary
Proper cell function relies on sorting transmembrane proteins. New research reveals novel cargo-adaptor interactions critical for clathrin-mediated endocytosis and protein recycling.
Area of Science:
- Cell Biology
- Molecular Biology
- Biophysics
Background:
- Transmembrane protein distribution is crucial for cellular function.
- The endocytic pathway utilizes clathrin-mediated endocytosis for protein transport.
- Adaptor proteins link cargo to clathrin, facilitating vesicle formation.
Purpose of the Study:
- To review recent advancements in understanding transmembrane protein sorting.
- To elucidate novel cargo-adaptor interactions in endocytic pathways.
- To explore the molecular mechanisms of cargo selection and vesicle maturation.
Main Methods:
- Structural biology studies.
- Biophysical analyses.
- Cell biological experiments.
Main Results:
- Identification of new types of cargo-adaptor interactions.
- Elucidation of molecular mechanisms regulating cargo selection.
- Insights into the regulation of vesicle maturation during endocytosis.
Conclusions:
- Recent studies have significantly advanced our knowledge of endocytic sorting.
- Understanding these mechanisms is key to comprehending cellular protein trafficking.
- This review synthesizes new findings with existing knowledge on endocytic sorting.
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