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Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

Rapid molecular strategy for orbivirus detection and characterization.

Gustavo Palacios¹, Chris Cowled, Ana V Bussetti

¹Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York 10032, USA. gp2050@columbia.edu

Journal of Clinical Microbiology

|April 1, 2011

Summary

A new consensus reverse transcription (RT)-PCR method aids in detecting diverse orbiviruses in various hosts. This technique simplifies orbivirus recognition and characterization through targeted polymerase gene analysis.

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Area of Science:

Virology
Molecular Biology
Genetics

Background:

Orbiviruses are a diverse group of viruses with a broad host range, including humans.
Detecting and characterizing orbiviruses is challenging due to their genetic diversity.

Purpose of the Study:

To develop a simple and effective method for the recognition and characterization of orbiviruses.
To overcome the limitations posed by orbivirus diversity in detection.

Main Methods:

A consensus reverse transcription (RT)-PCR method was designed targeting the polymerase gene.
Amplification products were analyzed using automated, web-based sequence analysis for phylogenetic assignment.

Main Results:

The developed RT-PCR method allows for the detection and characterization of a wide range of orbiviruses.
Phylogenetic assignment of detected orbiviruses is efficiently achieved through sequence analysis.

Conclusions:

This consensus RT-PCR approach provides a simplified strategy for orbivirus identification.
The method facilitates the recognition and phylogenetic placement of diverse orbiviruses, aiding in epidemiological studies.