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Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
Published on: September 13, 2018
Rapid molecular strategy for orbivirus detection and characterization
Gustavo Palacios1, Chris Cowled, Ana V Bussetti
1Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York 10032, USA. gp2050@columbia.edu
Journal of Clinical Microbiology
|April 1, 2011
Abstract:
Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.

