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A simple functional protein S assay using PROTAC.

P Han1, M Pradham

  • 1Haematology Division Laboratory Medicine, National University Hospital, Singapore.

Clinical and Laboratory Haematology
|January 1, 1990
PubMed
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A new functional assay for protein S, a crucial co-factor for protein C, was developed. This assay revealed unexpectedly high functional protein S levels in patients on warfarin therapy.

Area of Science:

  • Biochemistry
  • Hematology
  • Clinical Diagnostics

Background:

  • Protein S is an essential cofactor for the anticoagulant activity of activated protein C, particularly on factors FV and FVIII.
  • Currently, a readily available clinical functional assay for protein S is lacking.
  • Understanding protein S function is vital for hemostasis and thrombosis research.

Purpose of the Study:

  • To develop and validate a novel functional assay for protein S.
  • To determine functional protein S levels in healthy individuals and cord blood.
  • To investigate functional protein S levels in patients receiving long-term warfarin therapy.

Main Methods:

  • A functional protein S assay was developed utilizing the prolongation of Russell's viper venom (RVV) plasma clotting time.

Related Experiment Videos

  • Protein C activation was achieved using a PROTAC activator.
  • Functional protein S levels were measured in healthy individuals, cord blood samples, and patients on warfarin therapy.

    • Main Results:

    • The median functional protein S level in 46 healthy individuals was 82% (5-95th percentile: 60-120%).
    • Median functional protein S in 15 cord blood samples was 58% (35-71%).
    • Patients on long-term warfarin therapy (INR 1.1-3.9) exhibited a median functional protein S of 71% (40-101%), with no evidence of protein S decarboxylation.

    Conclusions:

    • The developed assay provides a functional measurement of protein S activity.
    • Functional protein S levels are lower in neonates compared to healthy adults.
    • Warfarin therapy does not appear to significantly reduce functional protein S levels, and protein S remains non-decarboxylated.