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Efficient transfer of interferon-induced virus resistance between human cells
The Journal of General Virology
|November 1, 1978
Summary
Virus resistance develops faster in mixed human cell cultures, driven by the quickest responding cell type. This interferon-mediated protection transfers more efficiently between human cells than between human and mouse cells, suggesting an amplified defense mechanism.
Area of Science:
- Virology
- Cell Biology
- Immunology
Background:
- Interferon induces virus resistance in cells.
- Cellular responses to interferon can vary.
- Understanding cell-to-cell communication in antiviral defense is crucial.
Purpose of the Study:
- To investigate the kinetics of interferon-induced virus resistance in mixed human cell cultures.
- To compare the efficiency of virus resistance transfer between homologous (human-human) and heterologous (human-mouse) cell types.
- To elucidate the mechanism of amplified antiviral protection in mixed cell populations.
Main Methods:
- Utilized two human cell lines (U and WISH) and mouse L cells.
- Employed two distinct interferon assay procedures.
- Tested resistance against vesicular stomatitis virus and Sindbis virus.
Main Results:
- The rate of interferon-induced virus resistance in mixed human cells is dictated by the fastest-responding cell type.
- Homospecific transfer of virus resistance (human-human) is significantly more efficient than heterospecific transfer (human-mouse).
- Lower donor-to-recipient cell ratios and faster transfer times were observed in homospecific transfers.
Conclusions:
- Interferon-mediated antiviral protection is amplified more effectively in mixtures of different human cells compared to human-mouse cell mixtures.
- Rapidly responding cells can influence slower-responding cells in mixed populations, potentially enhancing overall defense.
- This cell-cell communication mechanism likely plays a defensive role in vivo, amplifying interferon's protective effects.