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Related Experiment Video

Updated: Jun 3, 2026

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
08:24

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Published on: November 11, 2022

Caffeine can be used for oocyte enucleation.

Hui-Li Wang1, Zhong-Le Chang, Kun-Liang Li

  • 1College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City, People's Republic of China.

Cellular Reprogramming
|April 2, 2011
PubMed
Summary

Caffeine and MG132 offer safer alternatives for oocyte enucleation in cloning. These agents induce ooplasmic protrusions, leading to successful nuclear transfer, embryo development, and live births in goats, comparable to traditional methods.

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Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Animal Cloning

Background:

  • Oocyte enucleation is critical for nuclear transfer cloning.
  • Microtubule interrupters, while effective, can have adverse effects on oocyte development.
  • Safer chemical alternatives for enucleation are needed.

Purpose of the Study:

  • To evaluate caffeine and MG132 as less harmful agents for chemical-assisted oocyte enucleation.
  • To compare the efficacy of caffeine and MG132 with demecolcine and blind aspiration in goat, pig, and mouse oocytes.

Main Methods:

  • Goat, pig, and mouse oocytes were treated with caffeine or MG132 to induce ooplasmic protrusions.
  • Enucleation, cell fusion, and in vitro blastulation rates were assessed.
  • Pregnancy and live birth rates were monitored following nuclear transfer using caffeine-assisted enucleation.

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Last Updated: Jun 3, 2026

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
08:24

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Published on: November 11, 2022

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
09:16

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

Nuclear Transfer into Mouse Oocytes
14:17

Nuclear Transfer into Mouse Oocytes

Published on: November 30, 2006

Main Results:

  • Caffeine and MG132 induced ooplasmic protrusions in ~85% of goat oocytes, similar to demecolcine.
  • Enucleation, fusion, and blastulation rates were comparable between caffeine, MG132, and demecolcine treatments.
  • Live kids were born after caffeine-assisted enucleation in goats, with no significant difference in pregnancy or birth rates compared to demecolcine.

Conclusions:

  • Caffeine is a viable and effective agent for enucleation of goat and pig oocytes, yielding results comparable to demecolcine.
  • Caffeine-assisted enucleation supports successful embryo development and live births, offering a safer alternative.
  • Mouse oocytes showed limited response to caffeine and demecolcine, indicating species-specific efficacy.