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Related Experiment Video

Updated: Jun 3, 2026

Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
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Spontaneous decrease of CD14 cell surface expression in human peripheral blood monocytes ex vivo.

Herbert Bosshart1, Michael Heinzelmann

  • 1Department of Surgery, Zurich University Hospital, Switzerland. h_bosshart@bluewin.ch

Journal of Immunological Methods
|April 2, 2011
PubMed
Summary
This summary is machine-generated.

Bacterial lipopolysaccharide (LPS) affects monocyte CD14 expression ex vivo. This study reveals that cell culture conditions, not just LPS, influence CD14 levels, impacting how we interpret monocyte responses.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Monocyte CD14 (lipopolysaccharide receptor) surface expression shows a biphasic pattern ex vivo with continuous bacterial lipopolysaccharide (LPS) exposure.
  • This pattern is typically attributed solely to LPS effects, with limited consideration for cell culture artifacts.

Purpose of the Study:

  • To differentiate the effects of LPS exposure from cell culture conditions on monocyte CD14 surface expression ex vivo.
  • To re-evaluate the interpretation of previously observed biphasic CD14 expression patterns.

Main Methods:

  • Monocytes were cultured from diluted whole blood.
  • CD14 surface expression was analyzed in both LPS-treated and untreated samples under specific cell culture conditions.

Main Results:

  • LPS treatment induced CD14 surface expression in monocytes.
  • Untreated monocytes exhibited decreased CD14 surface expression during culture.
  • The observed biphasic pattern in prior studies may result from combined LPS induction and spontaneous CD14 downregulation.

Conclusions:

  • Cell culture conditions significantly influence monocyte CD14 expression ex vivo.
  • The spontaneous decrease of CD14 in untreated cells must be considered when analyzing LPS-induced changes.
  • Accurate interpretation of monocyte CD14 expression requires careful consideration of experimental culture parameters.